Objective: Present study designed to evaluate the pharmacological value of medicinal papaya seeds on quantitative hemoglobin estimation in conjunction with renal biochemistry tested on healthy rabbits.  Materials and Methods: This experimental and interventional study conducted on animal subdivide group, papaya seeds powder were given at dose 250mg & 500mg once a day subsequently laboratory evaluation was performed at 0, 15, 30, 45 days.  Result: Statistically proven incremental (7.8%, 11.4%) response in hemoglobin all tested groups noted. Increase in cumulative hemoglobin (g/dl) 11.2 ± 0.65, 10.9 ± 0.50 was noted as compared to baseline (p<0.05). Moreover Packed Cell Volume and Mean Corpuscular Hemoglobin Concentration were also showed increased value in test group B. Additionally group C also showed increase in Packed Cell Volume, Mean Corpuscular Volume and Mean Corpuscular Hemoglobin (p<0.05). Desirably Urea and Creatinine remained unchanged. Conclusion: Desirable clinical improvement of Hemoglobin, Packed Cell Volume, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin and Mean Corpuscular Hemoglobin Concentration was noted after 250/500mg of papaya powder seeds.